In vivo nucleic acid assemblies for spatial organization in Synthetic Biology
mardi 15 mai 2012 - 11:30-13:30
Harvard Medical School, Department of Systems Biology, 200 Longwood Avenue - Boston, USA
Lieu : Salle de Conférences Pierre Schaeffer Bâtiment 409
The ability to control spatial organization in cells is highly valuable. Clustering into micro-compartiments or onto scaffolds helps direct substrate flow in-between interacting proteins, limits cross-talk between signaling pathways, and generally increases specificity and yields of sequential metabolic reactions. De novo spatial organization could be achieved using engineered nanoscale polynucleotide architectures expressed by the cells and designed to template specific metabolic pathways. Here, we report the design and assembly of multi-dimensional RNA-based structures in vivo, and their use as scaffolds for the spatial organization of bacterial metabolism. We systematically characterized these assemblies, and used them to control the spatial organization of a biofuel-producing biochemical pathway, which resulted in a significant increase in yields as a function of scaffold architecture. Taken together, these results indicate that self assembling polynucleotide structures can be used to rationally construct functional architectures in vivo.
Contact : Marie-Joëlle VIROLLE