Mécanismes de régulation par les petits ARN bactériens : une approche génétique
mardi 24 janvier 2012 - 11:30-13:00
Centre de Génétique Moléculaire CNRS - Gif-sur-Yvette
Lieu : Salle de conférence, bâtiment 400.
Base pairing between regulatory RNAs and complementary sequences in messenger RNAs is a highly conserved mechanism controlling gene expression levels in all forms of life. In bacteria, the best-studied regulatory small RNAs (sRNAs) are encoded at separate locations from their target genes and interact with cognate mRNAs through short and imperfect stretches of complementarity. Positive or negative regulation by these trans-encoded RNAs often requires the participation of chaperon protein Hfq. Studying bacterial small regulatory RNAs with a formal genetic approach has long been thought a challenging, if not impossible, exercise ; virtually all major in vivo studies of these molecules have involved the use of multi-copy plasmid clones. Very successful in the early phase of small RNA discovery, multi-copy expression has its weaknesses and can lead to misinterpretation. In my presentation, I will describe some examples of sRNA-mediated regulation studied in our laboratory where use of old-fashioned single-copy chromosomal genetics has proven decisive in deciphering the regulatory mechanism to the molecular detail.
Contact : Philippe BOULOC