Integrative study of the basic mechanisms of transcription in yeast : the central role of the Mediator of activation
mardi 6 décembre 2011 - 11:30-12:30
Équipe régulation transcriptionnelle des génomes, IBITec-S, DSV CEA Saclay
Lieu : Salle de conférence, bâtiment 400.
The Mediator is a 25 subunits complex conserved in eukaryotes that stimulates the transcription by RNA polymerase (Pol) II of activated genes. We have investigated the mechanisms through which we Mediator exerts its function. Using knowledge of protein-protein interaction map, we investigated the role of interactions between the Mediator and other component of the transcription machinery to demonstrate that they are essential for the assembly of the pre-initiation complexes at activated genes. Moreover, we demonstrated that the Mediator is responsible in vivo of the recruitment of Pol II through a direct contact at essentially all genes in eukaryotes and thus plays a pivotal role in transcription activation in eukaryotes.
Contact : Sylvie HERMANN-LE DENMAT